Home Print this page Email this page Users Online: 108
Home About us Editorial board Search Ahead of print Current issue Archives Submit article Instructions Subscribe Contacts Login 
Year : 2013  |  Volume : 1  |  Issue : 2  |  Page : 83-86

In vitro susceptibilities of fungal isolates against amphotericin B and voriconazole in Aspergillus keratitis: A comparative study

1 Dr Rajendra Prasad Centre for Ophthalmic Sciences, Ocular Microbiology, All India Institute of Medical Sciences, New Delhi, India
2 Department of Biostatistics, All India Institute of Medical Sciences, New Delhi, India
3 Cornea and Refractive Surgery, All India Institute of Medical Sciences, New Delhi, India

Correspondence Address:
Gita Satpathy
Professor of Ocular Microbiology, Dr Rajendra Prasad Centre for Ophthalmic Sciences, All India Institute of Medical Sciences, New Delhi - 110 029
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/2320-3897.112175

Rights and Permissions

Objectives: To study the susceptibility patterns of clinical isolates of Aspergillus species to amphotericin B and voriconazole. Materials and Methods: Fifty isolates of Aspergillus species (21 A. flavus, 14 A. fumigatus and 15 A. niger) from keratitis cases were tested for their susceptibilities to amphotericin B and voriconazole by a broth microdilution method, standardized in our laboratory. The relative MIC 50 units were calculated from the absolute MIC 50 (μg/ml) upon topical prescription dose in gm/100 ml. Results: The MIC values of amphotericin B for A. fumigatus and A. niger ranged between 0.2 mg/L to 6.25 mg/L (MIC 50; 1.56 mg/L and MIC 90; 6.25 mg/L) and for A. flavus between 0.78-6.25 mg/L (MIC 50 and MIC 90; 6.25 mg/L). MICs of voriconazole for A. fumigatus were in the ranges of 0.1 to 0.2 mg/L, for A. niger 0.05 to 0.2 mg/L (MIC 50 and MIC 90; 0.2 mg/L for both fungi) and against A. flavus 0.05 to 0.39 mg/L (MIC 50 ; 0.2 mg/L and MIC 90 ; 0.39 mg/L). The relative MIC 50 of voriconazole against all the three Aspergillus species was 0.002, compared to those of amphotericin B which were 0.104 against A. fumigatus and A. niger and 0.416 against A. flavus. Conclusion: Voriconazole had lower MIC values and relative MICs than amphotericin B against Aspergillus species, the commonest fungi causing keratitis in this part of the country. Combined with its reported higher bio-availability, it appears far superior to amphotericin B for the treatment of fungal keratitis.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded330    
    Comments [Add]    

Recommend this journal